タンパク質マイクロアレイの技術導入動向

As protein arrays have become more consistent and reliable, scientists have begun to incorporate them into their proteomics experiments. The technologys ability to detect protein expression more quickly and easily than traditional approaches has begun to transform the dream of characterizing an organisms entire collection of proteins (i.e., proteome) into reality through high throughput analysis. While there is tremendous excitement about the potential of protein arrays to further our understanding about protein function, there are also concerns over frustrating technical limitations.

Based on a 39-question survey of scientists in the field of proteomics, Protein Microarrays: Technology Adoption & Utilization provides suppliers with insights into the types of products required to support this research. In order to benchmark the current state of the market for protein arrays, this report details researchers  experimental parameters, current and future levels of sample throughput and choice of supplier. It reveals respondents  satisfaction with their protein arrays, reagents and instrumentation, and determines the product attributes that are of the greatest importance, as well as areas where they perform above or below expectations. Additionally, the report explores why some proteomics researchers opt not to use protein arrays and what factors could influence scientists to begin using them.

By using this report to understand the actual experiences, needs and preferences of researchers, suppliers of protein arrays, arraying supplies and related equipment will be better able to make refinements to existing products to appeal to the diverse needs of their target market and extend the use of their protein array products into additional laboratories.

The major objectives of this report are as follows:

• Identify the key experimental parameters of protein microarray technology for both current and future users.
• Ascertain the reasons why researchers utilize protein microarrays.
• Identify factors that might induce researchers reluctant to adopt the technology to use protein microarrays.
• Determine current levels of throughput and estimate change in throughput over the next 12 months.
• Identify the preferred suppliers of protein microarray slides, products for self-printing of arrays and microarray readers.
• Measure respondents  levels of satisfaction with key attributes of protein microarrays and microarray readers.
• Determine the factors that influence respondents  decisions to choose one supplier over another for protein microarrays and readers.
• Understand respondents  expectations for future innovations in protein microarray technology.

Section 1. Analysis and Interpretation of Survey Results

Strategic Overview

• Executive Summary
• Key Findings
• Study Objectives

Competing Technologies

• Protein Microarray Adoption Rates
• Spotlight: Nonuser Technology Profile
• Market Segment Technology Preferences
• Value of Technology Performance

Experimental Parameters

• Protein Microarray Content
• Protein Classes
• Protein Microarray Density
• Whole Proteome Arrays
• Protein Microarray Format
• Protein Microarray Throughput

Market Dynamics: Protein Microarrays

• Preferred Suppliers of Self-Print Protein Microarray Reagents and/or Instrumentation
• Microarray Reagents and/or Instrumentation Performance Attributes
• Preferred Suppliers of Commercial Protein Microarrays
• Commercial Protein Microarray Performance Attributes

Market Dynamics: Protein Microarray Readers

• Preferred Suppliers of Protein Microarray Readers
• Protein Microarray Reader Performance Attributes
• Obstacles and Opportunities
• Technology Improvements

Conclusions

Section 2. Study Methodology and Demographics

• Methodology
• Definition of Terms
• Demographics
• Questionnaire

Section 3. Presentation of Survey Data

Research Focus

• Primary focus of research
• Primary source of proteins
• Proteomics-based technologies regularly employed
• Current protein array use

Future Plans

• Cost per protein for alternate proteomics-based technologies
• Willingness to pay more for wider dynamic range
• Willingness to pay more for increased reproducibility
• Willingness to pay more for increased sensitivity
• Factors that would influence non-users of protein arrays to begin use or begin use sooner

Experimental Parameters

• Types of protein interactions studied
• Types of applications for protein microarrays
• Specific protein classes studied
• Primary organism of interest for a whole proteome array
• Proteins printed per array
• How many replicates printed on arrays
• Samples analyzed per array
• Samples analyzed per month
• Expected change in throughput per month
• Microarray cost per protein
• Willingness to pay more for arrays with a two-fold increase in
• proteins printed per array
• Willingness to pay more for arrays with a ten-fold increase in
• proteins printed per array
• Willingness to pay more for a whole proteome array
• Most problematic step in protein microarray experiments

Protein Microarray Printing

• Use of commercial protein microarrays
• Primary reason for using commercial microarrays
• Suppliers of protein microarrays
• Primary supplier of reagents and instrumentation
• Satisfaction with features of reagents and instrumentation from primary supplier
• Overall satisfaction with reagents and instrumentation from primary supplier
• Primary supplier of protein microarrays
• Satisfaction with features of microarrays from primary supplier
• Overall satisfaction with microarrays from primary supplier
• Supplier factors in protein microarray purchasing decision

Protein Microarray Detection

• Primary brand of microarray reader used
• Satisfaction with features of this microarray reader
• Overall satisfaction with this microarray reader
• Supplier factors in microarray reader purchasing decision

Future Directions

• Areas of improvement in protein microarray technology

Section 4. Appendices

• Insights and Perspectives
• Cross-Tabulations of Survey Data
• Other Recent Publications
• About BioInformatics
• Our Valued Clients