プロテオミクスの市場：2003年版

Overview

Based on data collected from over 540 researchers, lab directors, and executives involved in proteomics research-including participants from 13 of the largest 15 and 19 of the largest 25 pharmaceutical companies-the CHI Proteomics Survey Report 2003 provides detailed analysis and insight into key strategic and tactical forces driving the development and application of proteomics technologies.

The report examines all major aspects of proteomics, including protein separation, ultrafiltration, 1D and 2D gel electrophoresis, liquid chromatography, capillary electrophoresis, mass spectrometry, protein informatics, protein arrays, protein quantification, protein localization, and protein-protein interactions.

Survey participants provided key information on current and projected spending, their intention to adopt or drop specific proteomics technologies and applications, detailed the advantages and disadvantages of current and emerging technological approaches to proteomics, and more.

With 70 detailed areas of analysis and over 100 color graphs, all contained within a concise 50 page report, the Proteomics Survey Report 2003 is a uniquely efficient, primary-data grounded source of insight into the current and future state of proteomics research. (See table of contents for specific areas of analysis.)

Methodology

Cambridge Healthtech Institute (CHI) conducted its Proteomics 2002 survey in mid-2002, and recruited a total of 542 survey participants. Survey respondents were personally invited to participate, based on CHI' s knowledge of their interest and work in proteomics research. Survey participants were also ensured that their participation would be kept strictly confidential, in an effort to encourage completely frank responses to often sensitive questions. (See Appendix for list of survey participant organizations, and titles and departments.)

The survey was conducted on-line, with real-time validation of responses, and responses were gathered in a SQL database for further tabulation and analysis. The survey was comprised of thirteen discrete sections, covering a wide range of proteomics-related research activities, and respondents were encouraged to participate in those sections most relevant to their work and experience. As a result, the number of respondents participating in each section of the survey varies. Accordingly, any reference in this report to a number or proportion of respondents is intended to reference the number of substantive respondents to the particular survey question under discussion, unless otherwise noted.

TABLE OF CONTENTS

I. METHODOLOGY AND DEMOGRAPHICS

Methodology

Q020. Respondent Organization Types

Q030. Respondent Organization Revenues, FTEs, and Market Capitalization

Q040. Respondent Position Within Organization

II. PROTEOMICS GENERALLY

Q050. Proteomics Spending to Step Up in 2003

Q060. Majority Pursuing Multiple Proteomics Research Areas

Q067. Key to Future of Proteomics: Protein Antibody Chips, 2D/Mass Spec, and MDLC

Q070. Sample Prep the Key Bottleneck in Proteomics Research

Q080. System-Wide Proteomics Research Most Common

III. PROTEIN SEPARATION

Q090. Liquid Chromatography, Gels Remain the Dominant Separation Technologies

Q100. Separation Techniques Possess Clear Advantages & Disadvantages

Q110. Protein Separation Spending to Step Up in 2003

Q120. Adoption of Separation Technologies Likely in 2003

Q130. Differential Protein Expression Studies Most Common Reason for Protein Separation

IV. ULTRAFILTRATION

Q150. Millipore Leading Source for Ultrafiltration Equipment

Q160. Strong Growth in Demand Projected for Ultrafiltration

Q170. Key Unmet Need: Greater Variety of Molecular Weight Cut-Off Points

Q180. Ultrafiltration Spending to Step Up in 2003

V. 1D and 2D GEL ELECTROPHORESIS

Q190. 1D Gel Electrophoresis Maintains Edge over 2D

Q200. SDS-PAGE is the Dominant 1D Gel Material

Q210. SDS-PAGE is the Dominant 2D Gel Material

Q220. Robotics Used in Minority of 1D Gel Processing

Q230. Robotics Used in Minority of 2D Gel Processing

Q240. Non-Fluorescent Dyes Dominate Gel Staining

Q250. Majority of Gels Used Solely for Image-Based Analysis

Q260. Spots per Gel Subject to Further Physical Analysis

Q270. 1D Gels Run Far More Frequently than 2D Gels

Q280. Substantial Increase in Use of 1D/2D Gels Expected

Q290. Increased Automation, Improved Resolution are Key Unmet Needs in Gel Electrophoresis

Q300. Respondents Typically Use Gel-Related Products from Three Vendors

V. 1D and 2D GEL ELECTROPHORESIS

Q310. Increased Automation and Improved Resolution/Sensitivity are Key Unmet Needs in Image Analysis

Q320. Gel Electrophoresis Spending to Step Up in 2003

Q322. Gel Consumables Spending to Step Up in 2003

Q324. Gel Equipment Spending to Step Up in 2003

Q326. Imaging-Related Product Spending to Step Up in 2003

VI. LIQUID CHROMATOGRAPHY

Q330. Reverse Phase and Affinity LC Most Commonly Used Methods

Q340. Large Increases Forecast for Use of Liquid Chromatography

Q350. Resolution Cited as Both Advantage and Disadvantage of Liquid Chromatography

Q360. Five Most Common LC Setups Account for Half of Proteomics-Related Chromatography

Q370. Six LC Setups to Account for Majority of Increased Use in Proteomics

Q410. Affinity/Reverse Phase Chromatography Most Likely to be Adopted

Q420. Respondents Applying MS to LC Separations Do So Frequently

Q430. LC Spending to Step Up in 2003

Q440. Bio-Rad and Applied Biosystems Leading Sources for LC Equipment and Supplies

VII. CAPILLARY ELECTROPHORESIS (CE)

Q450. Respondents Using CE Do So Frequently

Q460. Large Majority of Respondents Using CE Expect to Increase Use

VIII. MASS SPECTROMETRY

Q490. MALDI-TOF Dominant, Followed by ESI-QTOF

Q500. Liquid Chromatography and Gel Electrophoresis Most Common pre-MS Separation Techniques

Q510. MS Ionization Methods Compete on Speed, Ease of Sample Prep, and Ease of Interface with LC and MS

Q530. Sensitivity and Accuracy By Far the Most Important Attributes of MS Setup

Q550. MALDI-TOF, ESI-ION TRAP Lead List of Setups to be Acquired

Q570. Number of Spectra Generated per Month to Increase 300% in 2002-2003

Q590. Phosphorylation the Most Commonly Studied Post-Translational Modification

Q610. MALDI-TOF and MALDI-TOF-TOF Lead List of MS Setups to be Acquired

Q620. Respondents Typically Use MC-Related Products from Two Vendors

Q640. Premium Placed on Integrated MS Platforms Sourced from Single Vendor

Q650. Proteomics-related MS Study Spending to Step Up in 2003

Q660. Proteomics-related MS Equipment Spending to Step Up in 2003

IX. PROTEIN INFORMATICS

Q670. SWISS-2DPAGE the Most Commonly Used 2D Gel Database

Q690. Mascot the Most Commonly Used Peptide Mass Database

Q710. SWISS-PROT the Most Commonly Used Protein Sequence Database

Q740. One-Third of Respondents Plan to Substantially Increase Spending on 2D Gel Databases in 2002-2003

Q750. Protein Sequence Database Spending to Step Up in 2003

X. PROTEIN ARRAYS

Q760. Expression and Protein-Protein Interaction Studies are Most Common Uses of Protein Arrays

Q770. Use of Protein Arrays Dominated by "Home-Grown" Arrays

Q790. Protein Array Spending to Step Up in 2003

XI. PROTEIN QUANTIFICATION

Q830. Gels and Lowry/BCA/Bradford Assays Most Common Protein Quantification Methods

XII. PROTEIN LOCALIZATION

Q860. Immuno and Centrifugation/Fractionation Most Common Techniques in Protein Localization

Q870. Membrane-Bound Proteins Most Common Localization Targets

XIII. PROTEIN-PROTEIN INTERACTIONS

Q950. Yeast Two-Hybrid Dominant Technique in Studying Protein-Protein Interactions

Q960. Transient/Stable Transfection Dominant Measure in Mammalian Protein Interaction Studies

XIV APPENDIX

A. Survey Participant Organizations

B. Survey Participant Titles and Departments