次世代シーケンシング：1000ドルゲノムの科学的、商業的影響

A growing number of companies and academic groups are reporting impressive progress in commercializing new sequencing platforms that offer orders of magnitude of improved throughput and cost. The prospect of routine, personalized genome sequencing is suddenly within reach, not to mention spectacular advances in a host of related fields.

This new report from CHI Insight Pharma Reports describes and assesses these developing technologies and their applications:

• Pyrosequencing
• Sequencing by synthesis
• Supported oligonucleotide detection (SOLiD)
• Single-molecule sequencing
• Nanopore sequencing
• Optical trapping

Next-Generation Sequencing: Scientific and Commercial Implications of the $1000 Genome also includes the results and analysis of a quantitative Web survey of the views, practices, and plans of individuals at life science organizations involved with gene sequencing.

The applications covered in this report include:

• Personal genomics
• The Cancer Genome Atlas
• Evolutionary genomics
• Environmental genomics

The report also describes the efforts and progress of the companies that have become leaders in the next-generation sequencing race, including:

• Applied Biosystems
• GE Healthcare
• Helicos BioSciences
• Illumina/Solexa
• Reveo
• Roche/454 Life Sciences
• VisiGen Biotechnologies

Next-Generation Sequencing: Scientific and Commercial Implications of the $1000 Genome is an essential tool for individuals involved in R&D or commercialization of next-generation sequencing technologies.

Table of Contents

A BRIEF HISTORY OF GENOME SEQUENCING TECHNOLOGY AND MILESTONES

• Dideoxy (Sanger) Sequencing
• Automated DNA Sequencing
• Genome Wars
• The $1,000 Genome

BEYOND SANGER SEQUENCING: NEXT-GENERATION SEQUENCING TECHNOLOGIES

• Sequencing-by-Synthesis/Pyrosequencing
• Sequencing-by-Synthesis
• Illumina
• GE Healthcare
• Intelligent Bio-Systems
• SOLiD (Supported Oligonucleotide Detection)
• Storage
• Polony Sequencing
• Single-Molecule Sequencing
• VisiGen
• Pacific Biosciences
• Nanopore Sequencing
• Optical Trapping
• Nanoscale Sanger Sequencing

APPLICATIONS OF NEXT-GENERATION SEQUENCING

• The $1,000 Genome/Personal Genomics
• The Cancer Genome Atlas
• Evolutionary Genomics
• Environmental Genomics
• Gene Regulation

EXPERT INTERVIEWS WITH TECHNOLOGY INNOVATORS

• Tony Smith, PhD, Illumina
• Susan Hardin, PhD, VisiGen
• Jay Shendure, MD, PhD, University of Washington, Seattle
• Kevin McKernan, ABI
• Stanley N. Lapidus, Helicos

NEXT-GENERATION COMPANIES

• Applied Biosystems
• GE Healthcare
• Helicos BioSciences
• Illumina/Solexa
• Intelligent Bio-Systems
• Pacific Biosciences
• Reveo
• Roche/454 Life Sciences
• VisiGen

CHI INSIGHT PHARMA REPORTS- NEXT-GENERATION SEQUENCING SURVEY- FEBRUARY 2007

APPENDIX

• NHGRI RESEARCH GRANTS
  • $1,000 Genome Grants (August 2005)
  • $100,000 Genome Grants

References

Company Index with Web Addresses

• Table 2.1. Next-Generation Sequencing Technologies
• Table 2.2. Features of Major Next-Generation Sequencing Platforms

• Figure 2.1. SOLiD: 2 Base Pair Encoding Using 4 Dyes
• Figure 2.2. SOLiD: Example of Decoding
• Figure 2.3. Apparatus for Single-Molecule Analysis Using Zero-Mode Waveguides
• Figure 6.1. Survey Respondents by Sector
• Figure 6.2. Primary Involvement in Next-Generation Sequencing
• Figure 6.3. Application of Next-Generation Sequencing
• Figure 6.4. Organisms Sequenced Using New Platform(s)
• Figure 6.5. Active Use of Next-Generation Sequencing Systems
• Figure 6.6. Most Promising Platform
• Figure 6.7. Chief Application of Next-Generation Sequencing
• Figure 6.8. Areas of Disease Research to Benefit from Next-Generation Sequencing
• Figure 6.9. Chief Technical Concerns about Next-Generation Sequencing
• Figure 6.10. Assessment of Current Platform Costs
• Figure 6.11. Cost of Full Human Genome Sequencing by 2010
• Figure 6.12. Dominant Platform
• Figure 6.13. Future Role of Sanger (Capillary) Sequencing
• Figure 6.14. Opinions Concerning GINA